2eyq

About this Structure
2EYQ is a 2 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

The crystallographic asymmetric unit of the solved structure contains 2 monomers of Mfd (residues 2-1147 of 1151 for one molecule and 5-1147 of the other), five HEPES molecules, and the sulfate ions.

Size exclusion chromatogaphy indicates that the protein exists as a monomer in solution.

The monomer colored as an N→C rainbow similar to Figure 1A of the article describing the structure. .

The domains in the structure of Mfd: The domains all shown and colored similar to Figure 1B and Figure 2 of the article describing the structure.
 * D1a
 * D2 (UvrB homology module)
 * D1b
 * D3
 * D4 (RNAP Interacting Domain)
 * Translocation module (D5 and D6)
 * D7 (handle)

Domains labeled and colored and displayed similar to Figure 1B and Figure 2 of the article describing the structure. Unlabeled. [ Note: the following view generates a substantial surface which may take several minutes to calculate. Use the one above as an alternative unless you are willing to spend the time. ] Domains labeled and represented much more similar to Figure 1B. .

The translocation module shown in the context of the Mfd displayed as in Figure 3A of the article describing the structure.

The seven ATPase/helicase motifs in the translocation domain are highlighted in red. See figure 3B.

<scene name='2eyq/Walkera/3'>Walker A motif of Mfd displayed similar to Figure 3B of the article describing the structure.

<scene name='2eyq/Figure3c/3'>TRG motif and motif VI indicated similar to Figure 3C of the article describing the structure.

Reference
Page originally seeded by OCA on Mon Feb 16 12:00:00 2009